Cypermethrin elicited responses in heat shock protein and feeding in Caenorhabditis elegans

Heat shock proteins (Hsp) are a family of stress proteins, which are elicited in response to a variety of stressors in organisms. The objective of this study was to explore the potential of cypermethrin, a synthetic pyrethroid insecticide, on induction of small heat shock proteins (Hsp16) and feeding response in transgenic Caenorhabditis elegans PC72 (Hsp16-LacZ). A concentration-dependent inhibition in feeding was evident in worms exposed to cypermethrin (31%, 46% and 56% at 5, 10, 15 mM, respectively) beyond 4h while marked induction of heat shock protein-16 expression was evident after 12h exposure (as evident from quantitative analysis). Maximum expression of Hsp16 was observed throughout the body of the worms 24h after exposure similar to that evident in the worms exposed to heat shock at 30 degrees C. These data suggest that cypermethrin possesses the potential to induce Hsp16 as well as inhibit feeding in C. elegans at non-lethal concentrations. C. elegans (PC72) thus could serve as a convenient model to study the early toxic effects of xenobiotics.