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Related Experiment Video

Updated: Jun 13, 2026

Murine Dermal Fibroblast Isolation by FACS
06:04

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Published on: January 7, 2016

Corneal myofibroblast generation from bone marrow-derived cells.

Flavia L Barbosa1, Shyam S Chaurasia, Alicia Cutler

  • 1Cole Eye Institute, The Cleveland Clinic, 9500 Euclid Ave., Cleveland, OH 44195, USA.

Experimental Eye Research
|April 27, 2010
PubMed
Summary

In this study, bone marrow-derived cells were found to develop into myofibroblasts, which cause corneal haze after phototherapeutic keratectomy (PTK). The majority of these myofibroblasts originated from bone marrow, not corneal cells.

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Area of Science:

  • Ophthalmology
  • Cell Biology
  • Regenerative Medicine

Background:

  • Corneal stromal haze after phototherapeutic keratectomy (PTK) is associated with myofibroblast presence.
  • The origin of these myofibroblasts, specifically whether they derive from bone marrow or resident corneal cells, remains unclear.

Purpose of the Study:

  • To investigate if bone marrow-derived cells can differentiate into myofibroblasts in the corneal stroma following irregular PTK.
  • To determine the contribution of bone marrow cells to myofibroblast formation and subsequent corneal haze.

Main Methods:

  • C57BL/6J-GFP chimeric mice were generated via bone marrow transplantation.
  • Irregular PTK was performed on one eye of GFP chimeric mice.
  • Immunocytochemistry was used to identify alpha-smooth muscle actin (SMA)-positive myofibroblasts and green fluorescent protein (GFP)-expressing bone marrow-derived cells.

Main Results:

  • At 4 weeks post-PTK, corneas exhibited corneal haze, with a significant increase in SMA+GFP+ myofibroblasts compared to SMA+GFP- myofibroblasts.
  • The majority of myofibroblasts identified were positive for both SMA and GFP, indicating derivation from bone marrow.
  • A significant population of SMA-GFP+ cells, likely inflammatory cells, were also observed.

Conclusions:

  • In this mouse model, bone marrow-derived cells are the primary source of myofibroblasts following irregular PTK.
  • These myofibroblasts contribute to the development of corneal stromal haze.
  • While bone marrow cells are the main source, the study cannot definitively exclude contributions from resident keratocytes or corneal fibroblasts.