Related Experiment Video
Updated: Jun 13, 2026

MR Molecular Imaging of Prostate Cancer with a Small Molecular CLT1 Peptide Targeted Contrast Agent
Published on: September 3, 2013
Cyclic bisbibenzyls induce growth arrest and apoptosis of human prostate cancer PC3 cells
Ai-hui Xu1, Zhi-min Hu, Jian-bo Qu
1Department of Biochemistry and Molecular Biology, School of Medicine, Shandong University, Ji-nan 250012, China.
Aim:
To investigate the cytotoxic effects of four cyclic bisbibenzyls, Riccardin C (Ric), Pakyonol (Pak), Marchantin M (Mar), and Plagiochin E (Pla) against chemoresistant prostate cancer PC3 cells.
Methods:
Cell growth was assayed by MTT method, and apoptotic related protein Bcl-2 and Bax, poly(ADP-ribose) polymerase (PARP) were examined by Western blotting. Cell cycle and apoptosis of PC3 cells were evaluated with flow cytometry and morphologic examinations.
Results:
The four compounds inhibited proliferation and elicited cell death in a dose- and time-dependent manner with IC(50) values of 3.22 micromol/L for Ric, 7.98 micromol/L for Pak, 5.45 micromol/L for Mar, and 5.99 micromol/L for Pla, respectively. Furthermore, exposed to these chemicals caused a decrease in the antiapoptotic protein Bcl-2 and an increase in proapoptotic Bax expression. PARP cleavage and caspase-3 activity were also observed.
Conclusion:
The results suggest that cyclic bisbibenzyls could be used for the development of novel therapeutic chemicals against prostate cancer.
Related Concept Videos
Inhibition of Cdk Activity
Inhibition of CDK Activity
Negative Regulator Molecules
Positive Regulator Molecules

