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Related Concept Videos

Carrier-Mediated Transport01:06

Carrier-Mediated Transport

Carrier-mediated transport is a pivotal process in drug absorption, particularly for lipid-insoluble drugs, and encompasses facilitated diffusion and active transport. Facilitated diffusion allows drugs to move along their concentration gradient without energy expenditure, while active transport utilizes ATP to drive drug movement against this gradient.
Active transport involves two types of membrane-spanning transporters: uptake and efflux. Uptake transporters are expressed in the small...
ABC Transporters: Exporter01:31

ABC Transporters: Exporter

ATP-binding cassette or ABC transporter is the largest superfamily of integral membrane proteins. The transporters have transmembrane-binding domains (TMDs) and nucleotide-binding domains (NBDs). The TMDs are specific to their substrates, whereas the NBDs are similar to engines that complete ATP hydrolysis to complete the substrate transport. They can be full transporters consisting of two TMDs and NBDs, half transporters with one TMD and NBD, while some encoded with a single TMD or NBD are...
Pharmacogenetics of Drug Transporters: P-Glycoprotein and Solute Carrier Transporters01:16

Pharmacogenetics of Drug Transporters: P-Glycoprotein and Solute Carrier Transporters

The pharmacogenetics of drug transporters is increasingly recognized as a critical factor influencing interindividual variability in drug absorption, distribution, and elimination. These membrane-bound proteins regulate drugs' movement across cellular barriers by actively pumping them out (efflux) or facilitating their uptake (influx). Among the major transporter families, ATP-binding cassette (ABC) and solute carrier (SLC) transporters play particularly prominent roles. Genetic polymorphisms...
The Significance of Membrane Transport01:44

The Significance of Membrane Transport

The transport of solutes across the cell membrane is essential for metabolic processes, like maintaining cell size and volume, generating the action potential, exchanging nutrients and gases, etc. Membrane transport can be either passive or active. It can be simple diffusion, facilitated, or mediated transport aided by transport proteins such as transporters and channels.
Transporters facilitate either an active or passive movement of solutes. They can allow a single-molecule transport down its...
Active Transport01:14

Active Transport

Active transport is a critical biological process that allows cells to move solutes against an electrochemical gradient. This process requires direct energy input and is characterized by its selectivity, saturability, and susceptibility to competitive inhibition.
Primary active transporters, like Na+, K+ and -ATPase, directly utilize ATP to move ions across the membrane. These transporters play significant roles in various physiological processes. For instance, Na+, K+ and -ATPase maintain...

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Related Experiment Video

Updated: Jun 13, 2026

Models and Methods to Evaluate Transport of Drug Delivery Systems Across Cellular Barriers
18:57

Models and Methods to Evaluate Transport of Drug Delivery Systems Across Cellular Barriers

Published on: October 17, 2013

Methods to evaluate transporter activity in cancer.

Takeo Nakanishi1, Douglas D Ross, Keisuke Mitsuoka

  • 1Department of Membrane Transport and Biopharmaceutics, School of Pharmaceutical Sciences, Kanazawa University, Kanazawa, Japan.

Methods in Molecular Biology (Clifton, N.J.)
|April 27, 2010
PubMed
Summary

Plasma membrane transporters influence drug absorption and excretion. Targeting these transporters in cancer cells offers potential for improved anticancer drug delivery and diagnostics.

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Area of Science:

  • Biochemistry and Molecular Biology
  • Pharmacology
  • Oncology

Background:

  • Plasma membrane transporters are crucial for cellular nutrient uptake and xenobiotic efflux.
  • These transporters significantly impact the absorption, distribution, and excretion of anticancer drugs.
  • Differential expression of transporters in cancer cells presents therapeutic and diagnostic opportunities.

Purpose of the Study:

  • To review methodologies for analyzing the activity of drug transport-involved transporters.
  • To highlight the role of transporters in anticancer drug efficacy and pharmacokinetics.
  • To explore the potential of transporters as targets for enhanced cancer therapy.

Main Methods:

  • Focus on methodologies for assessing transporter activity.
  • Discussion of techniques to analyze transporter-mediated drug transport.
  • Review of studies demonstrating transporter roles in drug disposition.

Main Results:

  • Transporter activity analysis is key to understanding drug pharmacokinetics.
  • Differential transporter expression in cancer impacts drug response.
  • Knowledge of transporter function enables targeted drug delivery and improved bioavailability.

Conclusions:

  • Analyzing transporter activity is essential for optimizing anticancer drug therapy.
  • Transporters represent promising targets for novel cancer treatment strategies.
  • Understanding transporter roles can lead to more effective and safer cancer drug development.