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Photocontrol of coiled-coil proteins in living cells

Fuzhong Zhang1, Katharina A Timm, Katja M Arndt

  • 1Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, M5S 3H6 Canada.

Angewandte Chemie (International Ed. in English)
|April 27, 2010
PubMed
Abstract

No abstract available in PubMed .

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Protein Dynamics in Living Cells

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Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...