Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Acute ischemic stroke, active malignancy and non-bacterial thrombotic endocarditis: an exploratory prospective observational study.

Scientific reports·2026
Same author

Ventilation strategies and outcomes after out-of-hospital cardiac arrest: protocol for a pre-planned sub-analysis of the STEPCARE trial.

BMJ open·2026
Same author

Temporal dynamics of neuroplasticity and neurodegeneration in the central auditory system following noise-induced hearing loss: a multimodal imaging and histological study.

Acta neuropathologica communications·2026
Same author

Striking a balance: A proposed benefit assessment matrix for ethical animal research.

Neuroscience applied·2026
Same author

Early Prediction of Neurological Outcome After Cardiac Arrest-Rationale and Design of the Prospective International Observational EARLY-NEURO, a STEPCARE Substudy.

Acta anaesthesiologica Scandinavica·2026
Same author

Measuring cerebral glucose metabolism by chemical exchange-sensitive spin-lock (CESL) MRI of 2-deoxy-D-glucose in rodents.

PloS one·2026

Related Experiment Video

Updated: Jun 13, 2026

Quantification of Neurovascular Protection Following Repetitive Hypoxic Preconditioning and Transient Middle Cerebral Artery Occlusion in Mice
09:48

Quantification of Neurovascular Protection Following Repetitive Hypoxic Preconditioning and Transient Middle Cerebral Artery Occlusion in Mice

Published on: May 4, 2015

Elevating intracranial pressure reverses the decrease in deoxygenated hemoglobin and abolishes the post-stimulus

Martina Füchtemeier1, Christoph Leithner, Nikolas Offenhauser

  • 1Department of Experimental Neurology and Center for Stroke Research, Charité - Universitätsmedizin Berlin, Charitéplatz 1, 10098 Berlin, Germany.

Neuroimage
|April 28, 2010
PubMed
Summary

Intracranial pressure (ICP) elevation alters brain signaling, potentially invalidating BOLD fMRI brain mapping. Studies show ICP affects deoxy-hemoglobin levels, suggesting BOLD signal changes are vascular, not metabolic.

More Related Videos

2-Vessel Occlusion/Hypotension: A Rat Model of Global Brain Ischemia
09:29

2-Vessel Occlusion/Hypotension: A Rat Model of Global Brain Ischemia

Published on: June 22, 2013

Pre-Chiasmatic, Single Injection of Autologous Blood to Induce Experimental Subarachnoid Hemorrhage in a Rat Model
09:14

Pre-Chiasmatic, Single Injection of Autologous Blood to Induce Experimental Subarachnoid Hemorrhage in a Rat Model

Published on: June 18, 2021

Related Experiment Videos

Last Updated: Jun 13, 2026

Quantification of Neurovascular Protection Following Repetitive Hypoxic Preconditioning and Transient Middle Cerebral Artery Occlusion in Mice
09:48

Quantification of Neurovascular Protection Following Repetitive Hypoxic Preconditioning and Transient Middle Cerebral Artery Occlusion in Mice

Published on: May 4, 2015

2-Vessel Occlusion/Hypotension: A Rat Model of Global Brain Ischemia
09:29

2-Vessel Occlusion/Hypotension: A Rat Model of Global Brain Ischemia

Published on: June 22, 2013

Pre-Chiasmatic, Single Injection of Autologous Blood to Induce Experimental Subarachnoid Hemorrhage in a Rat Model
09:14

Pre-Chiasmatic, Single Injection of Autologous Blood to Induce Experimental Subarachnoid Hemorrhage in a Rat Model

Published on: June 18, 2021

Area of Science:

  • Neuroscience
  • Physiology
  • Medical Imaging

Background:

  • Blood-oxygen-level-dependent (BOLD) functional magnetic resonance imaging (fMRI) relies on detecting deoxygenated hemoglobin (deoxy-Hb) changes.
  • The impact of elevated intracranial pressure (ICP) on BOLD fMRI signal integrity and neurovascular coupling remains unclear.
  • ICP elevation offers a method to differentiate vascular from metabolic contributions to BOLD signal changes, particularly the post-stimulus undershoot.

Purpose of the Study:

  • To investigate how elevated intracranial pressure (ICP) affects neurovascular coupling and deoxy-hemoglobin (deoxy-Hb) signaling.
  • To determine if the BOLD post-stimulus undershoot is caused by vascular compliance or elevated cerebral metabolic rate of oxygen (CMRO(2)).

Main Methods:

  • Anesthetized rats underwent cranial window implantation over the somatosensory cortex.
  • Cerebral blood flow (CBF), cerebral blood volume (CBV), and deoxy-Hb were measured using laser Doppler flowmetry and optical spectroscopy during forepaw stimulation.
  • Intracranial pressure (ICP) was elevated via cerebrospinal fluid infusion, while neuronal activity was monitored via somatosensory evoked potentials.

Main Results:

  • Neurovascular coupling was not abolished by ICP elevation.
  • The expected decrease in deoxy-Hb during stimulation was reduced at moderate ICP (14 mmHg) and reversed at high ICP (28 mmHg).
  • The BOLD post-stimulus undershoot amplitude decreased with ICP elevation, and CMRO(2) did not increase, indicating a passive vascular origin.

Conclusions:

  • The validity of BOLD fMRI for brain mapping may be compromised under conditions of elevated ICP.
  • The BOLD post-stimulus undershoot is a passive vascular phenomenon, not directly reflecting elevated metabolic demand.
  • These findings highlight the importance of considering ICP in the interpretation of fMRI data.