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Related Concept Videos

mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
Phase II Reactions: Methylation Reactions01:17

Phase II Reactions: Methylation Reactions

Methylation is a phase II biotransformation process involving the attachment of a methyl group to a substrate. Enzymes known as methyltransferases orchestrate this reaction.
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Transcription Attenuation in Prokaryotes

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Updated: Jun 13, 2026

DNAzyme-dependent Analysis of rRNA 2&#8217;-O-Methylation
09:12

DNAzyme-dependent Analysis of rRNA 2’-O-Methylation

Published on: September 16, 2019

Yeast targets for mRNA methylation.

Zsuzsanna Bodi1, James D Button, Donald Grierson

  • 1School of Biosciences, Plant Sciences Division, University of Nottingham, Sutton Bonington, Loughborough LE12 5RD, UK.

Nucleic Acids Research
|April 28, 2010
PubMed
Summary

N(6)-Methyladenosine (m(6)A) is a widespread mRNA modification in yeast sporulation. This study identifies m(6)A in key meiotic genes, suggesting its regulatory role in yeast development.

Area of Science:

  • Molecular Biology
  • Epigenetics
  • Yeast Genetics

Background:

  • N(6)-Methyladenosine (m(6)A) is a prevalent mRNA modification in eukaryotes.
  • m(6)A levels increase during sporulation in Saccharomyces cerevisiae.
  • The methyltransferase Ime4 is linked to meiosis initiation, but its targets and m(6)A function remain unclear.

Purpose of the Study:

  • To investigate the distribution and function of m(6)A during yeast sporulation.
  • To identify specific mRNA targets of m(6)A methylation.
  • To elucidate the role of m(6)A in regulating meiotic development.

Main Methods:

  • Quantification of m(6)A levels in sporulating yeast mRNA.
  • Development of an antibody-based method to detect m(6)A-containing transcripts.

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Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response
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  • Analysis of m(6)A modification in key meiotic regulator genes (IME1, IME2, IME4).

    • Main Results:

    • Substantial m(6)A levels were found in the GpA context in sporulating yeast mRNA.
    • Approximately 50% of transcripts may contain m(6)A during meiosis, distributed across all mRNA sizes.
    • Transcripts of IME1, IME2, and IME4 were identified as methylated, with m(6)A localized to the 3'-end of IME2.

    Conclusions:

    • m(6)A is a widespread mRNA modification in yeast meiosis, not limited to specific transcripts.
    • Methylation of key meiotic genes suggests a regulatory role for m(6)A in controlling developmental pathways.
    • IME4-mediated m(6)A modification likely influences developmental decisions leading to meiosis.