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CKS1B nuclear expression is inversely correlated with p27Kip1 expression and is predictive of an adverse survival in

Hong Chang1, Nan Jiang, Hua Jiang

  • 1Department of Laboratory Hematology, University Health Network, 200 Elizabeth Street, 11E-413 Toronto, ON, M5G 2C4. hong.chang@uhn.on.ca

Haematologica
|April 28, 2010
PubMed
Abstract

Insights

Cyclin kinase subunit 1B (CKS1B) amplification and nuclear protein expression are linked in multiple myeloma, indicating a poor prognosis. Immunohistochemistry for CKS1B can predict adverse outcomes and aid in patient risk stratification.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • CKS1B, a cell cycle regulator, is amplified at chromosome 1q21 in multiple myeloma.
  • 1q21 amplification is an adverse prognostic factor, but its correlation with CKS1B protein expression is unknown.
  • Understanding this relationship is crucial for multiple myeloma patient stratification.

Purpose of the Study:

  • To correlate nuclear CKS1B protein expression with 1q21 amplification status.
  • To investigate the relationship between CKS1B expression, p27(Kip1) levels, and patient survival.
  • To evaluate CKS1B immunohistochemistry as a predictive tool for multiple myeloma.

Main Methods:

  • Immunohistochemistry was used to assess CKS1B and p27(Kip1) nuclear expression in 94 newly diagnosed multiple myeloma bone marrow biopsies.
  • Interphase fluorescence in situ hybridization (FISH) analyzed CKS1B gene status (1q21 amplification) in clonal plasma cells.
  • Correlation analyses were performed between CKS1B expression, 1q21 amplification, p27(Kip1), and overall survival.

Main Results:

  • 1q21 amplification was detected in 38% of patients; CKS1B protein expression was observed in 39%.
  • A strong correlation existed between 1q21 amplification and CKS1B protein expression (P<0.0001).
  • CKS1B expression inversely correlated with p27(Kip1) (P<0.0001) and was associated with significantly shorter overall survival (44.5 vs. 89.3 months, P<0.0001).

Conclusions:

  • CKS1B nuclear protein expression strongly correlates with 1q21 amplification in multiple myeloma.
  • Immunohistochemistry for CKS1B is a rapid and effective method to predict 1q21 amplification.
  • CKS1B expression serves as a valuable biomarker for adverse outcome prediction and risk stratification in multiple myeloma.

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