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Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells
Published on: May 1, 2020
HDAC2 promotes eIF4E sumoylation and activates mRNA translation gene specifically
Xiang Xu1, Jaya Vatsyayan, Chenxi Gao
1Department of Pharmacology and Chemical Biology, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA.
The Journal of Biological Chemistry
|April 28, 2010
Summary
Histone deacetylase 2 (HDAC2) promotes protein synthesis by enhancing sumoylation of eukaryotic translation initiation factor 4E (eIF4E). This novel function regulates cell proliferation and apoptosis, independent of its known deacetylase activity.
Area of Science:
- Molecular Biology
- Epigenetics
- Cancer Biology
Background:
- Histone deacetylases (HDACs) primarily function as transcription corepressors by removing acetyl groups from histones.
- The role of HDACs beyond transcriptional regulation is an emerging area of research.
Purpose of the Study:
- To investigate the non-epigenetic functions of HDAC2.
- To explore the involvement of HDAC2 in mRNA translation.
Main Methods:
- Assays to measure protein sumoylation.
- Analysis of eukaryotic translation initiation factor 4E (eIF4E) modification.
- Assessment of eukaryotic initiation factor 4F (eIF4F) complex formation.
- Gene expression analysis of eIF4E-responsive genes.
Main Results:
- HDAC2 promotes the sumoylation of eIF4E, independent of its deacetylase activity.
- HDAC2-mediated eIF4E sumoylation enhances the formation of the active eIF4F translation complex.
- HDAC2 regulates the protein synthesis of genes critical for cell proliferation and apoptosis prevention.
Conclusions:
- HDAC2 possesses an uncharacterized sumoylation-promoting activity.
- HDAC2 plays a novel role in regulating cap-dependent mRNA translation.
- HDAC2's function in translation impacts cellular processes like proliferation and survival.
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