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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.

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Related Experiment Video

Updated: Jun 13, 2026

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays
09:58

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Published on: June 23, 2022

Immunoassays in microfluidic systems.

Alphonsus H C Ng1, Uvaraj Uddayasankar, Aaron R Wheeler

  • 1Institute for Biomaterials and Biomedical Engineering, University of Toronto, 164 College St., Toronto, ON M5S 3G9, Canada.

Analytical and Bioanalytical Chemistry
|April 28, 2010
PubMed
Summary
This summary is machine-generated.

Microfluidic systems have advanced immunoassays through miniaturization. This review covers microfluidic immunoassay configurations, fluid handling, multiplexing, and label-free detection, highlighting future potential.

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Biotechnology

Background:

  • Immunoassays are crucial for diagnostics and research.
  • Miniaturization offers advantages like reduced sample volume and faster analysis.
  • Microfluidic platforms enable significant advancements in immunoassay technology.

Purpose of the Study:

  • To review developments in microfluidics-based immunoassays since 2000.
  • To consolidate knowledge on immunoassay configurations, fluid handling, multiplexing, and detection techniques.
  • To highlight the progress and future prospects of microfluidic immunoassays.

Main Methods:

  • Literature review of microfluidics-based immunoassays published since 2000.
  • Categorization of studies based on immunoassay configurations.
  • Analysis of fluid handling modalities, multiplexing strategies, and detection methods.

Main Results:

  • Overview of diverse immunoassay configurations implemented on microfluidic chips.
  • Summary of key fluid handling techniques employed in microfluidic immunoassays.
  • Discussion of advancements in multiplexed immunoassays and label-free detection methods.

Conclusions:

  • Microfluidic immunoassays have shown continuous improvement and innovation.
  • The integration of microfluidics offers enhanced performance for immunoassays.
  • The field holds significant promise for future diagnostic and analytical applications.