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Assays for the Identification of Novel Antivirals against Bluetongue Virus

Assays for the Identification of Novel Antivirals against Bluetongue Virus

Published on: October 11, 2013

Genetically engineered structure-based vaccine for bluetongue disease.

¹Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, United Kingdom.

Veterinaria Italiana

|April 28, 2010

Summary

New virus-like particle (VLP) vaccines offer a safer alternative to live-attenuated vaccines for bluetongue (BT) disease. These VLP vaccines are highly immunogenic and provide broad protection against multiple BT virus (BTV) serotypes without the risk of genetic reassortment.

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Assays for the Identification of Novel Antivirals against Bluetongue Virus

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Published on: October 11, 2013

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Homogeneous Glycoconjugate Produced by Combined Unnatural Amino Acid Incorporation and Click-Chemistry for Vaccine Purposes

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Published on: December 19, 2020

Area of Science:

Veterinary Virology
Vaccine Technology
Immunology

Background:

Current bluetongue (BT) vaccines are live-attenuated, posing risks of vaccine failure and genetic mutation.
The segmented RNA genome of bluetongue virus (BTV) allows for gene reassortment, potentially creating novel virulent strains.
A need exists for safer, more effective BTV vaccines devoid of harmful genetic material.

Purpose of the Study:

To develop and evaluate virus-like particle (VLP) vaccines as a safe and effective alternative to live-attenuated BTV vaccines.
To assess the immunogenicity and protective efficacy of BTV VLPs in sheep against virulent BTV challenge.
To determine the breadth of protection offered by a VLP cocktail against multiple BTV serotypes.

Main Methods:

Generation of BTV virus-like particles (VLPs) using protein expression technology.
Vaccination of sheep with BTV VLPs under natural environmental conditions.
Challenge of vaccinated sheep with virulent BTV strains to assess protection.
Evaluation of immune responses and protection duration post-vaccination.

Main Results:

BTV VLPs were highly immunogenic in sheep, eliciting protective immune responses.
Vaccinated sheep were protected against virulent BTV challenge up to 15 months post-immunization.
A cocktail of five VLPs demonstrated cross-protection against related heterologous BTV serotypes.
VLPs are rapidly producible and easily purified, offering advantages for large-scale vaccine manufacturing.

Conclusions:

BTV VLP vaccines represent a safe and promising alternative to live-attenuated vaccines.
VLPs induce robust and long-lasting immunity, protecting against virulent BTV infection.
A VLP-based strategy can provide broad protection against multiple BTV serotypes, addressing the challenge of viral diversity.