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Updated: Jun 13, 2026

Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro
Published on: April 21, 2023
Antibacterial nanoparticle monolayers prepared on chemically inert surfaces by cooperative electrostatic adsorption
Sabil Huda1, Stoyan K Smoukov, Hideyuki Nakanishi
1Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois 60208, USA.
Abstract:
Cooperative electrostatic adsorption (CELA) is used to deposit monolayer coatings of silver nanoparticles on relatively chemically inert polymers, polypropylene, and Tygon. Medically relevant components (tubing, vials, syringes) coated by this method exhibit antibacterial properties over weeks to months with the coatings being stable under constant-flow conditions. Antibacterial properties of the coatings are due to a slow release of Ag(+) from the particles. The rate of this release is quantified by the dithiol-precipitation method coupled with inductively coupled plasma optical emission spectrometer (ICP-OES) analysis.

