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[Screen of a membrane protein molecule from Vero cells capable of binding Japanese encephalitis virus(JEV)]
Yan Li1, Ying Shi, Tian-bing Ding
1Department of Microbiology, Fourth Military Medical University, Xi'an 710032, China.
Aim:
Identification of membrane protein molecules Vero cells binding JEV.
Methods:
Membrane protein extract was subject to co-immunoprecipitation (Co-IP) with JEV, and identified by mass (MS) spectrometry. The binding between specific extract protein and JEV was measured by MS, flow cytometry (FCM) and immunofluorescence assay (IFA).
Results:
Heat shock protein 90 beta (HSP90 beta) was identified by Co-IP and MS, and its binding JEV activity was confirmed by FCM and IFA.
Conclusion:
HSP 90 beta from Vero cells membrane could bind JEV.
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