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Related Experiment Video

Updated: Jun 13, 2026

Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages
06:52

Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages

Published on: May 21, 2018

Inflammasome and IL-1beta-mediated disorders.

Hal M Hoffman1, Alan A Wanderer

  • 1Division of Allergy and Immunology, University of California at San Diego and Rady Children's Hospital of San Diego, La Jolla, CA 92093, USA. hahoffman@ucsd.edu

Current Allergy and Asthma Reports
|April 29, 2010
PubMed
Summary

The NLRP3 inflammasome and interleukin-1beta are crucial in disease development. Targeting interleukin-1beta shows promise for treating various inflammatory disorders.

Area of Science:

  • Immunology
  • Molecular Biology
  • Pathogenesis

Background:

  • The NLRP3 inflammasome is an intracellular multiprotein complex.
  • It mediates the release of pro-inflammatory cytokines, notably interleukin-1beta (IL-1β).
  • Its activation is triggered by exogenous pathogens and endogenous danger signals.

Purpose of the Study:

  • To review the role of the NLRP3 inflammasome and IL-1β in disease pathogenesis.
  • To highlight the therapeutic potential of IL-1β targeted therapies.

Main Methods:

  • Evidence synthesis from human genetics studies.
  • Analysis of human ex vivo mononuclear cell responses.
  • Review of in vivo and in vitro murine models.

Main Results:

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Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells
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Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells

Published on: May 22, 2014

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Published on: May 21, 2018

Accurate and Simple Measurement of the Pro-inflammatory Cytokine IL-1β using a Whole Blood Stimulation Assay
06:29

Accurate and Simple Measurement of the Pro-inflammatory Cytokine IL-1β using a Whole Blood Stimulation Assay

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Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells
09:04

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells

Published on: May 22, 2014

  • The NLRP3 inflammasome and IL-1β are confirmed key players in the pathogenesis of several inherited and complex diseases.
  • Targeted IL-1β therapies have demonstrated successful proof-of-concept in certain disorders.

Conclusions:

  • The NLRP3 inflammasome and IL-1β are significant therapeutic targets for inflammatory diseases.
  • Further exploration of inflammasome-mediated pathways may reveal new treatment strategies for additional diseases.