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Updated: Jun 13, 2026
Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
Published on: June 1, 2011
Nanoengineered electrically contacted enzymes on DNA scaffolds: functional assemblies for the selective analysis of
Gilad Mor-Piperberg1, Ran Tel-Vered, Johann Elbaz
1Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
Abstract:
A DNA construct consisting of a nucleic acid template, (1), on which a nucleic acid-modified glucose oxidase (GOx), (3), was hybridized by cooperative bridging of the T-Hg(2+)-T units, and a nucleic acid-functionalized ferrocene, (5), was directly hybridized on a Au electrode. The resulting nanostructure revealed bioelectrocatalytic activities, where the ferrocene units mediated electron transfer between the redox center of the enzyme and the electrode. The bioelectrocatalytic functions of the system are regulated by the concentration of Hg(2+) ions, which controls the content of the enzyme associated with the DNA template by means of the T-Hg(2+)-T bridging units. This phenomenon allowed the amperometric detection of Hg(2+) ions at a detection limit 1 x 10(-10) M with impressive selectivity.
