Simultaneous determination of L- and D-T4 using a sequential injection analysis/sensors system

Area of Science:

• Analytical Chemistry
• Biomedical Engineering
• Pharmaceutical Analysis

Background:

• Accurate quantification of L- and D-thyroxine (T4) is crucial for pharmaceutical quality control.
• Existing methods may lack the precision, efficiency, or flexibility required for simultaneous determination.
• On-line monitoring of T4 enantiomers in synthesis processes is desirable.

Purpose of the Study:

• To develop and validate a sequential injection analysis (SIA) system for the simultaneous determination of L- and D-T4.
• To utilize a combination of biosensor and immunosensor technologies for enantioselective detection.
• To assess the system's suitability for on-line process control in pharmaceutical manufacturing.

Main Methods:

• A sequential injection analysis (SIA) system was employed.
• Simultaneous detection of L- and D-T4 using an amperometric biosensor (for D-T4) and an immunosensor (for L-T4).
• Optimization of buffer selection and sensor integration for high precision and accuracy.

Main Results:

• The SIA system demonstrated high precision and accuracy for both L- and D-T4 determination.
• The system achieved lower consumption of samples and buffers compared to conventional methods.
• The developed method enabled reliable on-line monitoring with a frequency exceeding 30 samples per hour.

Conclusions:

• The SIA system with integrated biosensor and immunosensor is a robust and efficient tool for simultaneous L- and D-T4 analysis.
• This method is suitable for quality control of raw materials and pharmaceutical products.
• The system's on-line capability facilitates real-time synthesis process control.