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MicroRNA miR-155 inhibits bone morphogenetic protein (BMP) signaling and BMP-mediated Epstein-Barr virus reactivation

Qinyan Yin1, Xia Wang, Claire Fewell

  • 1Tulane Health Sciences Center, 1430 Tulane Avenue, New Orleans, LA 70112, USA.

Journal of Virology
|April 30, 2010
PubMed

Insights

MicroRNA miR-155 targets bone morphogenetic protein (BMP) signaling components, inhibiting BMP

Area of Science:

  • Molecular Biology
  • Oncology
  • Virology

Background:

  • MicroRNA miR-155 is upregulated in various human cancers, including lung, breast, and colon.
  • miR-155 is induced by Epstein-Barr virus (EBV) in B cells, and related microRNAs are encoded by other oncogenic herpesviruses.
  • Bone morphogenetic protein (BMP) signaling regulates cellular processes like differentiation and growth inhibition, and its dysregulation is linked to cancer.

Purpose of the Study:

  • To investigate the role of miR-155 in targeting BMP signaling components.
  • To determine the impact of miR-155 on BMP-mediated cellular processes and EBV reactivation.
  • To explore miR-155's influence on the antitumor effects of BMP signaling.

Main Methods:

  • Analysis of miR-155 targeting of BMP signaling components (SMAD1, SMAD5, HIVEP2, CEBPB, RUNX2, MYO10) in their 3' UTR.
  • Assessment of miR-155's effect on BMP-induced ID3 expression and EBV reactivation in EBV-positive B cells (Mutu I).
  • Evaluation of miR-155's impact on BMP-mediated p21 induction and cell growth inhibition in lung epithelial cells (A549).

Main Results:

  • miR-155 directly targets multiple BMP signaling mediators, inhibiting BMP2-, BMP6-, and BMP7-induced ID3 expression.
  • miR-155 inhibits BMP-mediated EBV reactivation in Mutu I cells and suppresses SMAD1/SMAD5 expression in A549 cells.
  • miR-155 reverses BMP-mediated cell growth inhibition and induction of the cyclin-dependent kinase inhibitor p21.

Conclusions:

  • miR-155 plays a significant role in modulating BMP-mediated cellular processes.
  • miR-155 regulates BMP-induced EBV reactivation, suggesting a link between viral latency and host signaling.
  • miR-155 inhibits the antitumor effects of BMP signaling, potentially contributing to cancer development and progression.

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