Multicenter collaborative study for standardization of Candida albicans genotyping using a polymorphic microsatellite...

Area of Science:

• Microbiology
• Genetics

Background:

• Microsatellite-based genotyping is crucial for Candida albicans.
• Fragment size determination in genotyping can vary between laboratories due to differing electrophoretic conditions.

Purpose of the Study:

• To assess the interlaboratory reproducibility of microsatellite-based genotyping for Candida albicans.
• To determine if an allelic ladder can standardize results across different labs.

Main Methods:

• Six laboratories participated in the study.
• An allelic ladder was provided to all participating laboratories.
• Microsatellite genotyping was performed on Candida albicans samples.

Main Results:

• Variations in fragment size determinations were observed between laboratories.
• Despite size variations, correct allele assignments were consistently achieved.
• The use of an allelic ladder facilitated accurate allele identification.

Conclusions:

• Microsatellite genotyping for Candida albicans can be standardized across laboratories.
• Allelic ladders are effective tools for ensuring accurate allele assignment.
• Standardized genotyping methods enhance the transportability and reliability of Candida albicans data.