Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Peripheral neuropathy after chronic endoneurial ischemia.

J T Sladky1, R L Tschoepe, J H Greenberg

  • 1Department of Neurology, University of Pennsylvania School of Medicine, Philadelphia.

Annals of Neurology
|March 1, 1991
PubMed
Summary

Creating arteriovenous shunts in rats reduced sciatic nerve blood flow by 50-75%. This chronic nerve ischemia caused significant functional deficits and mild structural changes without infarction.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Peri-infarct flow transients predict outcome in rat focal brain ischemia.

Neuroscience·2012
Same author

Voxel-level comparison of arterial spin-labeled perfusion MRI and FDG-PET in Alzheimer disease.

Neurology·2011
Same author

Clinical, electrophysiologic, and pathologic evidence for sensory abnormalities in ALS.

Neurology·2007
Same author

Practice parameter: immunotherapy for Guillain-Barré syndrome: report of the Quality Standards Subcommittee of the American Academy of Neurology.

Neurology·2003
Same author

Teaching in the field: the model of a one-day trip to an outreach clinic.

Seminars in neurology·2002
Same author

A new model of localized ischemia in rat somatosensory cortex produced by cortical compression.

Stroke·2001

Area of Science:

  • Neuroscience
  • Vascular Biology
  • Peripheral Nerve Research

Background:

  • Chronic regional nerve ischemia is a significant factor in peripheral nerve dysfunction.
  • Understanding the effects of reduced blood flow on nerve structure and function is crucial for developing therapeutic strategies.

Purpose of the Study:

  • To establish a reliable method for inducing chronic regional nerve ischemia in rats.
  • To investigate the functional and morphological consequences of sustained, non-infarctive endoneurial blood flow reduction in the sciatic nerve.

Main Methods:

  • A surgical technique involving proximal limb arteriovenous shunts was employed in rats.
  • Endoneurial blood flow was quantified using the iodoantipyrine method.
  • Nerve conduction velocities were measured to assess functional deficits.

Related Experiment Videos

  • Morphological examinations were conducted on ischemic nerves.

    • Main Results:

    • The arteriovenous shunt procedure successfully reduced distal sciatic nerve endoneurial blood flow by 50-75%.
    • A 25-30% decrease in nerve conduction velocity was observed within 2 weeks, persisting for up to 10 months.
    • Morphological analysis revealed structural abnormalities at nodes of Ranvier and mild axonal atrophy, but no segmental demyelination or axonal degeneration.

    Conclusions:

    • Reduced endoneurial blood flow, even without causing infarction, can lead to significant and persistent functional impairments in peripheral nerves.
    • The developed arteriovenous shunt model provides a valuable tool for studying the pathophysiology of chronic nerve ischemia.
    • These findings highlight the sensitivity of peripheral nerve function to sustained reductions in blood supply.