Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Potentiometry: Membrane Electrodes01:15

Potentiometry: Membrane Electrodes

Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at the...
Controlled-Potential Coulometry: Electrolytic Methods01:17

Controlled-Potential Coulometry: Electrolytic Methods

Controlled-potential coulometry, also known as potentiostatic coulometry, employs a three-electrode system in which the working electrode's potential is precisely regulated using a potentiostat. Platinum working electrodes are utilized for positive potentials, while mercury pool electrodes are favored for extremely negative potentials. The platinum counter electrode is separated from the analyte using a membrane or salt bridge to avoid interference in the analysis.
The chosen potential ensures...
Dialysis01:15

Dialysis

Dialysis is a diffusion-based purification process that separates analyte molecules from a complex matrix. This is accomplished by allowing molecules in the solution to pass through a semipermeable membrane into a liquid on the other side. The membrane is usually made of cellulose acetate or cellulose nitrate, and the second liquid must be miscible with the solution. Ions (e.g., chloride or sodium) or organic molecules (e.g., glucose) can pass through the membrane pores, which generally have...
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
Coulometry: Overview01:00

Coulometry: Overview

Coulometry is one of the rapid, most accurate, and precise analytical techniques that determine the quantity of an analyte by measuring the electrical charge needed for its complete electrolysis without using any analytical standards. The total charge passed during electrolysis correlates with the analyte amount by Faraday's laws of electrolysis. For accurate coulometric measurements, a charge equal to Faraday's constant multiplied by the number of electrons involved in the relevant...
Controlled-Current Coulometry: Overview01:27

Controlled-Current Coulometry: Overview

Controlled current coulometry, also known as amperostatic coulometry, is a technique used in electrochemical analysis to measure the quantity of a substance through the controlled passage of current. It involves the application of a constant current to an electrochemical cell containing the analyte of interest. As the current flows through the cell, the analyte undergoes a redox reaction at the electrode surface, resulting in a charge transfer. By monitoring the time required for a certain...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Overcoming Limitations for Ultrasensitive <i>In Situ </i>pH Measurements in Marine Waters while Maintaining Traceability to Primary Standards.

Chimia·2026
Same author

Symmetrical pH Electrochemical Cell Coupled to Constant Potential Coulometry for Improved Sensitivity and Precision: Part 1. Fundamental Considerations.

ACS measurement science au·2026
Same author

Symmetrical pH Electrochemical Cell Coupled to Constant Potential Coulometry for Improved Sensitivity and Precision: Part 2. Submersible Probe for In Situ Measurements.

ACS measurement science au·2026
Same author

Zero-current chronopotentiometry for wired biosensors.

Mikrochimica acta·2025
Same author

Let Us Talk about pH, the Confusing Pillar of Aqueous Systems.

ACS sensors·2025
Same author

Spatially Resolved Ion Sensing by Voltammetric Ion Transfer Microscopy.

JACS Au·2025

Related Experiment Video

Updated: Jun 13, 2026

Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique
09:18

Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique

Published on: May 3, 2015

Thin layer coulometry with ionophore based ion-selective membranes.

Ewa Grygolowicz-Pawlak1, Eric Bakker

  • 1Department of Chemistry, Nanochemistry Research Institute, Curtin University of Technology, Perth, WA 6845, Australia.

Analytical Chemistry
|May 1, 2010
PubMed
Summary
This summary is machine-generated.

This study introduces a novel thin-layer coulometric detection mode for ion-selective sensors. This calibration-free method offers robust calcium ion detection in solutions, ideal for challenging applications.

More Related Videos

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone
08:06

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone

Published on: February 23, 2017

Capturing the Interaction Kinetics of an Ion Channel Protein with Small Molecules by the Bio-layer Interferometry Assay
10:41

Capturing the Interaction Kinetics of an Ion Channel Protein with Small Molecules by the Bio-layer Interferometry Assay

Published on: March 7, 2018

Related Experiment Videos

Last Updated: Jun 13, 2026

Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique
09:18

Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique

Published on: May 3, 2015

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone
08:06

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone

Published on: February 23, 2017

Capturing the Interaction Kinetics of an Ion Channel Protein with Small Molecules by the Bio-layer Interferometry Assay
10:41

Capturing the Interaction Kinetics of an Ion Channel Protein with Small Molecules by the Bio-layer Interferometry Assay

Published on: March 7, 2018

Area of Science:

  • Electroanalytical Chemistry
  • Chemical Sensors
  • Membrane Science

Background:

  • Ion-selective electrodes (ISEs) typically require frequent recalibration.
  • Liquid ion-selective membranes (LISM) often face challenges with stability and in-situ recalibration.
  • Coulometric detection offers a potential pathway for calibration-free sensor design.

Purpose of the Study:

  • To demonstrate a thin-layer coulometric detection mode for ionophore-based LISM.
  • To develop a robust, calibration-free sensor for calcium ion detection.
  • To investigate the use of porous polypropylene tubing for coulometric sensing.

Main Methods:

  • A thin-layer cell was constructed using porous polypropylene tubing doped with membrane components.
  • The membrane contained dodecyl 2-nitrophenyl ether, ETH 500, calcium ionophore ETH 5234, and KTFPB cation-exchanger.
  • Coulometric measurements were performed by applying potentials relative to open circuit potentials and integrating current decays.

Main Results:

  • The developed sensor exhibited permselectivity and followed the Nernstian equation for open circuit potentials.
  • Exhaustive current decays were observed for calcium chloride concentrations from 10 to 100 µM.
  • Integrated charge showed a linear dependence on calcium ion concentration, enabling coulometric readout.

Conclusions:

  • A novel thin-layer coulometric detection mode for LISM has been successfully demonstrated.
  • The developed sensor is calibration-free and shows promise for robust calcium ion monitoring.
  • This approach is suitable for applications where in-situ recalibration is difficult.