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Related Concept Videos

General Transcription Factors01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...

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Related Experiment Video

Updated: Jun 13, 2026

High-throughput Purification of Affinity-tagged Recombinant Proteins
07:44

High-throughput Purification of Affinity-tagged Recombinant Proteins

Published on: August 26, 2012

Transcription factor IIH - the protein complex with multiple functions.

Z Mydlikova1, J Gursky, M Pirsel

  • 1Laboratory of Molecular Genetic, Cancer Research Institute, Slovak Acadmey of Sciences, Slovak Republic. zuzana.mydikova@savba.sk

Neoplasma
|May 1, 2010
PubMed
Summary

Transcription factor IIH (TFIIH) is a conserved protein complex vital for DNA transcription and repair. Mutations in TFIIH cause severe genetic disorders, highlighting its crucial role in human health.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Transcription factor IIH (TFIIH) is a highly conserved eukaryotic multi-protein complex.
  • Initially recognized for its role in basal transcription initiation and elongation.
  • Emerging evidence points to its involvement in DNA repair and cell cycle regulation.

Purpose of the Study:

  • To review the structure and functions of TFIIH.
  • To explore TFIIH's roles in transcription and DNA repair.
  • To discuss the association of TFIIH with human genetic disorders.

Main Methods:

  • Literature review of TFIIH research.
  • Analysis of TFIIH structure and function.
  • Correlation of TFIIH mutations with genetic disorders.

Main Results:

  • TFIIH plays critical roles in transcription initiation, DNA repair (nucleotide excision repair), and cell cycle regulation.
  • Mutations in TFIIH subunits are linked to xeroderma pigmentosum, Cockayne syndrome, and trichothiodystrophy.
  • The conserved structure of TFIIH across species underscores its fundamental biological importance.

Conclusions:

  • TFIIH is a multifunctional complex essential for maintaining genomic stability and cellular processes.
  • Understanding TFIIH function and dysfunction is crucial for diagnosing and potentially treating related genetic disorders.
  • Further research into TFIIH is warranted to fully elucidate its complex roles in health and disease.