Related Experiment Video
Updated: Jun 13, 2026
Virus-induced Gene Silencing (VIGS) in Nicotiana benthamiana and Tomato
Published on: June 10, 2009
An efficient in vitro-inoculation method for Tomato yellow leaf curl virus
Ayed M Al Abdallat1, Hmoud S Al Debei, Heba Asmar
1Department of Horticulture and Crop Science, Faculty of Agriculture, University of Jordan, Amman, Jordan. a.alabdallat@ju.edu.jo
Background:
Tomato yellow leaf curl virus (TYLCV) is a member of the family Geminiviridae, genus Begomovirus. To test the infectivity of TYLCV in tomato plants, an improved protocol for inoculation of in vitro-cultured tomato plants was developed.
Results:
A TYLCV isolate was cloned, sequenced and used to construct a 1.8-mer infectious clone. Three weeks old microshoots of TYLCV-susceptible tomato plants were inoculated with Agrobacterium tumefaciens harboring the infectious clone for the TYLCV isolate. After two weeks, the TYLCV symptoms started to appear on the in vitro-inoculated plants and the symptoms became more severe and pronounced eight weeks post-inoculation. The method was used efficiently to uncover the resistance mechanism against TYLCV in Solanum habrochaites accession LA 1777, a wild tomato known for its high resistance to whitefly and TYLCV.
Conclusions:
The reported in vitro-inoculation method can be used to screen tomato genotypes for their responses to TYLCV under controlled conditions and it will be a useful tool for better understanding of the TYLCV biology in tomato plants.
