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siRNA - Small Interfering RNAs

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...

Small interfering RNAs (siRNA)

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Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
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Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

RNA Interference

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Related Experiment Video

Updated: Jun 13, 2026

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Published on: January 15, 2015

Sustained small interfering RNA delivery by mesoporous silicon particles.

Takemi Tanaka¹, Lingegowda S Mangala, Pablo E Vivas-Mejia

¹Department of Nanomedicine and Biomedical Engineering, University of Texas Health Science Center at Houston, Texas, USA.

Cancer Research

|May 1, 2010

Summary

This study introduces a novel multistage vector for in vivo RNA interference (RNAi) delivery, achieving sustained gene silencing and reduced tumor growth in ovarian cancer models. The system demonstrated safety and efficacy, offering potential for various diseases.

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Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery

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Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy

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Published on: June 21, 2013

Area of Science:

Biomedical Engineering
Molecular Biology
Oncology

Background:

In vivo RNA interference (RNAi) delivery faces challenges in safety, efficiency, and sustained systemic administration.
EphA2 oncoprotein overexpression is implicated in various cancers, including ovarian cancer.

Purpose of the Study:

To develop and validate a novel multistage vector for efficient and sustained in vivo delivery of small interfering RNA (siRNA).
To evaluate the therapeutic efficacy of the developed system in reducing tumor burden, angiogenesis, and proliferation in ovarian cancer models.

Main Methods:

A multistage vector composed of mesoporous silicon particles (S1MP) loaded with neutral nanoliposomes (dioleoyl phosphatidylcholine, DOPC) containing siRNA targeting EphA2.
Administration of the S1MP-siRNA-DOPC system via i.v. injection in orthotopic mouse models of ovarian cancer.
Assessment of EphA2 gene silencing, tumor burden, angiogenesis, cell proliferation, serum chemistries, and proinflammatory cytokines.

Main Results:

Sustained EphA2 gene silencing for at least 3 weeks following a single administration.
Significant reduction in tumor burden (54-57%), angiogenesis, and cell proliferation compared to control siRNA.
No significant adverse effects observed in serum chemistries or proinflammatory cytokine levels.

Conclusions:

The novel multistage siRNA delivery system provides a safe and effective method for sustained gene silencing in vivo.
This system shows therapeutic potential for ovarian cancer and other pathologies beyond neoplasms.
First in vivo therapeutic validation of this innovative multistage siRNA delivery approach.