Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Isolation of partial complementary DNA encoding human thromboxane synthase.

L H Wang1, K Ohashi, K K Wu

  • 1Department of Internal Medicine, University of Texas Medical School, Houston 77030.

Biochemical and Biophysical Research Communications
|May 31, 1991
PubMed
Summary

Researchers successfully cloned thromboxane synthase cDNA from human lung tissue. This advancement aids in understanding thromboxane A2

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Clinical study on the efficacy and safety of glaucoma drainage implants in the treatment of different types of glaucoma].

[Zhonghua yan ke za zhi] Chinese journal of ophthalmology·2024
Same author

Posttraumatic Stress, Anxiety, and Depression in COVID-19 Survivors.

East Asian archives of psychiatry : official journal of the Hong Kong College of Psychiatrists = Dong Ya jing shen ke xue zhi : Xianggang jing shen ke yi xue yuan qi kan·2022
Same author

Patients' Reports of Traumatic Experience and Posttraumatic Stress in Psychiatric Settings.

East Asian archives of psychiatry : official journal of the Hong Kong College of Psychiatrists = Dong Ya jing shen ke xue zhi : Xianggang jing shen ke yi xue yuan qi kan·2020
Same author

First Report of Paulownia Witches'-Broom Phytoplasma in China.

Plant disease·2019
Same author

First Report of a 16SrI-C Group Phytoplasma Associated With a Yellows-Type Disease Affecting Willow Plants in China.

Plant disease·2019
Same author

[A multicenter clinical study on 1 138 cases of invasive pneumococcal disease in children from 2012 to 2017].

Zhonghua er ke za zhi = Chinese journal of pediatrics·2018

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Enzymology

Background:

  • Thromboxane synthase is crucial for synthesizing thromboxane A2.
  • Thromboxane A2 significantly influences blood aggregation and blood vessel constriction.

Purpose of the Study:

  • To report the successful cloning of human thromboxane synthase cDNA.
  • To confirm the authenticity of the cloned cDNA sequence.

Main Methods:

  • Synthesized oligonucleotides based on purified human thromboxane synthase peptide sequences.
  • Employed polymerase chain reaction (PCR) with these oligonucleotides as primers.
  • Screened a human lung cDNA library to isolate the target complementary DNA (cDNA).

Main Results:

Related Experiment Videos

  • Successfully isolated a cDNA clone of thromboxane synthase.
  • The longest cDNA obtained measured 687 base pairs.
  • Deduced amino acid sequences from the cDNA matched the known peptide sequences, validating the clone.

    • Conclusions:

    • The successful cloning of human thromboxane synthase cDNA was achieved.
    • This molecular tool facilitates further research into thromboxane synthase function and regulation.