Related Experiment Video
Updated: Jun 13, 2026

An Acute Retinal Model for Evaluating Blood Retinal Barrier Breach and Potential Drugs for Treatment
Published on: September 13, 2016
Regulatory effect of histamine on the barrier function of intestinal mucosal
Ligeng Duan1, Xiaoli Chen, J W Alexander
1The Department of General Surgery, Huaxi Hospital, Medical Center, University of Sichuan, Chengdu 610041, China.
Objectives:
To investigate the regulatory effect of histamine on the barrier function of intestinal mucosal.
Methods:
The monolayer Caco-2 cell system in vitro and the model of hemorrhage infection in rats in vivo were established as experimental models. The amount of bacterial translocation was taken as an index of the effect of histamine and its receptor antagon, cimetidine on the intestinal mucosal barrier function.
Results:
(1) The in vitro experiment showed that after treatment with histamine, the CFU of Escherichia coli 075 invading into Caco-2 cells were much lower than that in the control group (P < 0.05). (2) The animal experiment showed that in the histamine group (hemorrhage infection rats treated with histamine), the average numbers of bacteria in the liver and lymph nodes were much lower than that in control group (P < 0.05). The mean bacterial number in the cimetidine group (hemorrhage infection rats treated both with histamine and cimetidine) was more than that in the histamine group, but without statistical signification (P > 0.05). But the rate of translocation to the liver between histamine group (37.5%) and cimetidine group (100%) was statistically different (P < 0.05)
Conclusion:
Small concentration of histamine can inhibit bacteria from entering epithelial cells and inhibit intestinal bacterial translocation.
Related Concept Videos
Mucosal Barrier of the Stomach
Within parietal cells, carbonic acid is first formed through the reaction of water and carbon dioxide. The dissociation of carbonic acid releases bicarbonate and hydrogen ions. The bicarbonate...
Acid Suppressive Drugs for Peptic Ulcer Disease: Histamine H2-Receptor Antagonists
Regulation of the Digestive System
The effectors in this regulation system are glands and smooth muscles. Activation of these...
Pathophysiology of Peptic Ulcer Disease: Mucosal Defense Factors
Pathophysiology of Peptic Ulcer Disease: Injurious Factors
In the antrum region, G cells secrete the gastrin hormone that binds to gastrin-cholecystokinin-B (CCK2) receptors on parietal and enterochromaffin-like (ECL) cells in the fundic glands. Simultaneously, the vagus nerve releases acetylcholine, which binds to M3...
Hormonal Regulation
