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Updated: Jun 13, 2026

A Cost-effective and Reliable Method to Predict Mechanical Stress in Single-use and Standard Pumps
Published on: August 5, 2015
Absolute measurement of internal volume changes inside the pulse damper: discrepancy between delay and dwell volumes
Eduard Rogatsky1, Zheng Zheng, Daniel Stein
1Analytical Core Laboratory, Institute for Clinical and Translational Research, Albert Einstein College of Medicine of Yeshiva University, Bronx, NY 10461, USA. eduard.rogatsky@einstein.yu.edu
Abstract:
We have developed a novel technique for the absolute determination of the mobile phase volume stored inside of a variable volume pulse damper at different pressures. Using an Agilent HPLC pulse damper we found a linear volume increase of approximately 1 microL/bar. We found that pump pressurization is a relatively slow process and takes approximately 1 min to reach 90% and takes approximately 2 min to reach 99% equilibration at flow rates below 1 mL/min. During pump pressurization, column flow rate will be less than the pre-set, since part of the mobile phase is retained inside of the pulse damper. During our experiments we observed a discrepancy between data obtained by UV techniques and direct absolute measurements. This difference can be explained by a fundamental difference between the gradient delay volume and dead (dwell) volume.
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Work Done During Volume Change
Consider a gas confined to a cylinder fitted with a movable piston at one end. If the gas expands from volume V1 to volume V2, it exerts a force on the piston, such that the piston moves by a distance dr.
The work done by the gas on the piston can be expressed as
Types of Damping
