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Analysis of CYP1A1 promoter function by transcription in vitro
L P Wen1, K W Jones, J P Whitlock
1Department of Pharmacology, Stanford University School of Medicine, California 94305.
Molecular Carcinogenesis
|January 1, 1991
Abstract:
We have analyzed the function of the CYP1A1 promoter using in vitro transcription. Using nuclear extracts from HeLa cells, we found that transcription from the promoter in vitro was constitutive. Transcription in vitro was not increased by prior exposure of the HeLa cells to the inducer 2,3,7, 8-tetrachlorodibenzo-p-dioxin nor by the inclusion of a dioxin-responsive enhancer in the DNA template. Analyses of mutants revealed that a TATAAA sequence and two recognition motifs for CCAAT box transcription factor/nuclear factor I contributed to the constitutive activity of the promoter in vitro.