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Updated: Jun 13, 2026
Method of Studying Palatal Fusion using Static Organ Culture
Published on: September 19, 2015
[Distraction osteogenesis for correction of cleft palate in rhesus-histological and fluorescent labeling study]
Yi Liu1, Gang Chen, Yan-shan Liu
1Department of Oral and Maxillofacial Surgery, the Stomatological Hospital of Tianjin Medical University, Tianjin 300070, China.
Objective:
To investigate the mechanism of new bone formation in the distraction osteogenesis (DO) for correction of cleft palate (CP) in rhesus.
Methods:
CP was created by operation in 23 rhesus. The CP was corrected with DO in 21 animals as experimental group. The distraction rate was 0.8 mm per day, two times a day. The bone fragments were fixed after cleft closure, every 3 animals were sacrificed to get specimen after 1, 2, 4, 6, 8, 12, 24 weeks of fixation. 6 days before sacrifice, tetracycline was administrated for labeling (30 mg/kg).
Results:
The hard and soft tissue def of fixation. At the same time, the bone volume and calcification between the distraction gap increased. The cleft in the control group could not b ect was successfully closed with DO by intramembrane osteogenesis. The new formed bone was remodeling and became maturation during the period e corrected spontaneously.
Conclusions:
The DO can successfully correct both the soft and hard tissue defect in CP by intramembrane osteogenesis. The fixation is important for remodeling and maturation of the new formed bone.
