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Related Experiment Video

Updated: Jun 13, 2026

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
12:36

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

Published on: February 16, 2014

Baculovirus for eukaryotic protein display.

Reingard Grabherr1, Wolfgang Ernst

  • 1University of Natural Resources and Life Sciences, Department of Biotechnology, Muthgasse 11, 1190 Vienna, Austria. reingard.grabherr@boku.ac.at

Current Gene Therapy
|May 4, 2010
PubMed
Summary
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Autographa californica nuclear polyhedrosis virus (AcMNPV) enables eukaryotic virus display by presenting foreign proteins on its surface. This baculovirus system facilitates immune responses, altered host specificity, and enhanced mammalian cell transduction for various applications.

Area of Science:

  • Biotechnology
  • Molecular Virology
  • Protein Engineering

Background:

  • Baculoviruses, specifically Autographa californica nuclear polyhedrosis virus (AcMNPV), are versatile platforms for eukaryotic surface display.
  • Various strategies exist for presenting target proteins on insect cells and budded virions using native or foreign fusion partners.

Purpose of the Study:

  • To explore strategies for presenting foreign proteins and peptides on the surface of insect cells and baculoviruses.
  • To highlight applications of baculovirus surface display, including vector design, library construction, and screening.

Main Methods:

  • Utilizing native baculovirus proteins (envelope, capsid) or foreign scaffolds (VSV-G, HA) as fusion partners for surface display.
  • Developing cloning strategies for constructing surface expression libraries.

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Last Updated: Jun 13, 2026

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  • Screening libraries for specific binding proteins with post-translational modifications.

    • Main Results:

    • Demonstrated feasibility of presenting diverse proteins and peptides on baculovirus surfaces.
    • Showcased applications in inducing immune responses and modifying baculovirus tropism for enhanced transduction.
    • Enabled selection of specific binding proteins from eukaryotic surface display libraries.

    Conclusions:

    • Baculovirus-based eukaryotic surface display offers a powerful system for presenting foreign proteins.
    • This technology has broad applications in vaccine development, targeted delivery, and biopharmaceutical discovery.
    • The described strategies facilitate the construction and screening of baculovirus surface display libraries.