Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Staphylococcal proliferation on skin models to investigate novel anti-infective treatments against dysbiosis.

Bioengineering & translational medicine·2026
Same author

HERA: a web server for host element reference-based aligner.

Nucleic acids research·2026
Same author

RegRegSEA: a web server for regulatory region set enrichment analysis of epigenomic data.

Nucleic acids research·2026
Same author

Probiotic Lactobacillus rhamnosus mitigates PBC-like features in Mcpip1-deficient mice via modulation of gut-liver crosstalk.

Biochimica et biophysica acta. Molecular basis of disease·2026
Same author

Benchmarking large language models for pathogen-disease classification in post-acute infection syndromes.

Briefings in bioinformatics·2026
Same author

mRNA Sequencing of Limbal Epithelial Cells and mRNA/miRNA Profiling of Limbal Stromal Cells in <i>PAX6</i>-Related Congenital Aniridia.

Cells·2026

Related Experiment Video

Updated: Jun 13, 2026

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
09:29

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

Deciphering the porcine intestinal microRNA transcriptome.

Soroush Sharbati1, Marc R Friedländer, Jutta Sharbati

  • 1Freie Universität Berlin, Institute of Veterinary Biochemistry, Oertzenweg 19b, 14163 Berlin, Germany. sharbati@zedat.fu-berlin.de

BMC Genomics
|May 4, 2010
PubMed
Summary

Researchers identified hundreds of novel microRNAs (miRNAs) in the porcine intestine, revealing their crucial role in intestinal function and development. This study significantly expands the known swine miRNAome, offering new insights into mammalian intestinal biology.

More Related Videos

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants
06:34

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants

Published on: January 21, 2020

Related Experiment Videos

Last Updated: Jun 13, 2026

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
09:29

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants
06:34

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants

Published on: January 21, 2020

Area of Science:

  • Genomics
  • Molecular Biology
  • Animal Science

Background:

  • Limited identification of microRNAs (miRNAs) in swine compared to humans.
  • Pigs are valuable models for human intestinal studies due to phylogenetic proximity.
  • miRNAs are critical regulators of intestinal development and linked to malignancy.

Purpose of the Study:

  • To identify and characterize novel microRNAs in the porcine intestine.
  • To comprehensively map the porcine intestinal miRNAome.
  • To investigate the role of miRNAs in intestinal function and development.

Main Methods:

  • Deep sequencing for initial miRNA identification.
  • miRDeep algorithm for miRNA precursor prediction.
  • Customized miRNA microarrays for comprehensive profiling across the entire intestine.
  • Hierarchical clustering to analyze expression patterns.

Main Results:

  • Identification of hundreds of novel porcine miRNAs in the intestine.
  • Discovery of 332 intestinal miRNAs, with 201 being novel.
  • Expression profiling revealed distinct miRNA subsets specific to different intestinal regions.
  • Genomic clustering of miRNA precursors identified on chromosomes 3 and 1.

Conclusions:

  • First comprehensive deciphering of the mammalian porcine intestinal miRNAome.
  • High number of novel miRNAs highlights their critical role in intestinal function.
  • Identified porcine miRNAs may have undiscovered orthologs in other mammals, including humans.