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Related Experiment Video

Updated: Jun 13, 2026

Assessing Transmissible Spongiform Encephalopathy Species Barriers with an In Vitro Prion Protein Conversion Assay
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Assessing Transmissible Spongiform Encephalopathy Species Barriers with an In Vitro Prion Protein Conversion Assay

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Ovine reference materials and assays for prion genetic testing.

Michael P Heaton1, Kreg A Leymaster, Theodore S Kalbfleisch

  • 1USDA, ARS, United States Meat Animal Research Center State Spur 18D, Clay Center, NE 68933, USA. mike.heaton@ars.usda.gov

BMC Veterinary Research
|May 4, 2010
PubMed
Summary

Identifying prion protein gene (PRNP) polymorphisms in sheep is crucial for accurate scrapie genetic testing. This study developed a DNA sequencing strategy to detect PRNP variations, aiding in scrapie eradication efforts.

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Area of Science:

  • Veterinary Genetics
  • Animal Breeding
  • Disease Resistance

Background:

  • Scrapie susceptibility in sheep is linked to prion protein gene (PRNP) variations.
  • Accurate DNA-based tests for PRNP codons are vital for scrapie eradication and disease resistance evaluation.
  • Undocumented PRNP polymorphisms can interfere with genetic testing fidelity.

Purpose of the Study:

  • To develop an adaptive DNA sequencing strategy for determining the ovine PRNP coding sequence.
  • To identify and characterize PRNP polymorphisms in U.S. sheep populations.
  • To establish reference panels for standardizing and improving scrapie genetic testing.

Main Methods:

  • An adaptive DNA sequencing strategy was employed to analyze the 771 bp PRNP coding sequence.

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Last Updated: Jun 13, 2026

Assessing Transmissible Spongiform Encephalopathy Species Barriers with an In Vitro Prion Protein Conversion Assay
11:41

Assessing Transmissible Spongiform Encephalopathy Species Barriers with an In Vitro Prion Protein Conversion Assay

Published on: March 10, 2015

Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material
09:50

Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material

Published on: September 29, 2017

Detection of Abnormal Prion Protein by Immunohistochemistry
06:38

Detection of Abnormal Prion Protein by Immunohistochemistry

Published on: May 5, 2023

  • The strategy incorporated known polymorphisms and an overlapping amplicon design to detect unknown variants.
  • Mass spectrometry multiplex assays were developed to genotype key PRNP codons (112, 136, 141, 154, 171).

    • Main Results:

    • A consensus PRNP sequence was generated for targeted U.S. sheep flocks.
    • DNA sequencing identified 16 previously unreported PRNP polymorphisms, including a novel L237P variant.
    • Reference tissues, DNA, and genotypes are publicly available.

    Conclusions:

    • Characterizing ovine PRNP polymorphisms is essential for effective scrapie genetic testing systems.
    • Reference DNA panels and identified polymorphisms support test development and certification.
    • This work contributes to expediting sheep scrapie eradication through improved genetic knowledge.