Ultrastructure of the siphonaceous green alga Halimeda cuneata, with emphasis on the cytoskeleton

Cytoplasm streaming is a fundamental process for the transport of molecules and organelles in plant cells. In vegetative filaments of the coenocytic green alga, Halimeda cuneata Hering, the spatial organisation of microtubules in the cytoplasmic layer, was observed under transmission electron microscopy. A cross section of a cortical filament shows a tubular cell wall enclosing a peripheral layer of cytoplasm with numerous chloroplasts, amyloplasts, nuclei, mitochondria and microtubules surrounding a small central vacuole. Towards the thallus medulla the central vacuole enlarges considerably and the cytoplasm becomes gradually reduced to a thin parietal layer, the number of organelles is reduced and the quantity of microtubules increases. Therefore, most of the thallus volume is occupied by the huge central vacuole which extends throughout the coenocytic filaments. Microtubules run longitudinally, being about 0.05 microm from each other. Some microtubules were observed in close association to cell organelles.