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Detection of Jeotgalicoccus spp. in poultry house air

Elena Martin1, Kerstin Fallschissel, Peter Kämpfer

  • 1Bundesanstalt für Arbeitsschutz und Arbeitsmedizin, Nöldnerstrasse 40-42, 10317 Berlin, Germany.

Systematic and Applied Microbiology
|May 4, 2010
PubMed

Insights

Jeotgalicoccus bacteria are widespread in poultry houses, making up a significant portion of airborne microbes. This study developed a method to quantify these bacteria in the air.

Area of Science:

  • Environmental microbiology
  • Poultry science
  • Bacterial genomics

Background:

  • Bioaerosols in poultry houses can harbor diverse microbial communities.
  • Understanding the composition of airborne bacteria is crucial for animal health and biosecurity.
  • Jeotgalicoccus species have not been extensively studied in poultry environments.

Purpose of the Study:

  • To investigate the prevalence and abundance of Jeotgalicoccus species in poultry house bioaerosols.
  • To develop a quantitative method for detecting Jeotgalicoccus cluster I (Jeot-cluster-I) bacteria.
  • To assess the proportion of Jeotgalicoccus spp. within total airborne microbial populations in poultry settings.

Main Methods:

  • Collection and analysis of bioaerosols from turkey, chicken, and duck houses.
  • 16S rRNA gene sequencing to identify bacterial populations.
  • Development of a group-specific PCR primer for Jeot-cluster-I.
  • Quantitative real-time PCR (qPCR) for bacterial enumeration.

Main Results:

  • Jeotgalicoccus-related sequences constituted 4-18% of 16S rRNA gene sequences in clone libraries.
  • Jeotgalicoccus halotolerans and J. psychrophilus were detected in all samples, forming Jeot-cluster-I.
  • qPCR revealed Jeotgalicoccus cell concentrations ranging from 10^4 to 10^6 cells/m^3 of air.
  • Jeotgalicoccus spp. represented 1-39% of total bacterial counts in the air.

Conclusions:

  • Jeotgalicoccus species are widely distributed in the poultry industry's airborne environments.
  • A significant proportion of airborne bacteria in poultry houses belong to the Jeotgalicoccus genus.
  • The developed qPCR method allows for effective quantification of these bacteria in poultry bioaerosols.

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