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Updated: Jun 13, 2026

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
Liquid chromatographic separation of pregabalin and its possible impurities with fluorescence detection after
Michal Dousa1, Petr Gibala, K Lemr
1Zentiva, as Praha, U Kabelovny 130, 102 37 Praha 10, Czech Republic. hplc@hplc.cz
Abstract:
A rapid procedure based on direct extraction and RP-HPLC separation of pregabalin and its possible impurities with fluorescence detection has been developed. The separation conditions and parameters of derivatization reaction for postcolumn derivatization of pregabalin with o-phtaldialdehyde/2-mercaptoethanol were studied. Purospher STAR RP-8e column with isocratic elution was employed. Fluorescence detection was performed at excitation and emission wavelength of 345 nm and 450 nm, respectively. The proposed method has an advantage of a simple sample pre-treatment and a quick and very sensitive HPLC method. The applicability of developed method was successfully verified during analysis of commercial samples of tablets of Lyrica (Pfizer, USA).
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