Related Experiment Video
Updated: Jun 13, 2026
13:31
Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis
Published on: October 31, 2014
Random mutagenesis of Clostridium cellulolyticum by using a Tn1545 derivative
Jean-Charles Blouzard1, Odile Valette, Chantal Tardif
1Centre National de la Recherche Scientifique, IFR88, UPR 9043, Laboratoire de Chimie Bactérienne, 31 Chemin Joseph Aiguier, 13402 Marseille, Cedex 20, France.
Applied and Environmental Microbiology
|May 4, 2010
Abstract:
Further understanding of the plant cell wall degradation system of Clostridium cellulolyticum and the possibility of metabolic engineering in this species highlight the need for a means of random mutagenesis. Here, we report the construction of a Tn1545-derived delivery tool which allows monocopy random insertion within the genome.
Related Concept Videos
01:16In vitro Mutagenesis
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
01:16In-vitro Mutagenesis
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.