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ROS mediate brassinosteroids-induced plant stress responses
Xiao-Jian Xia1, Zhixiang Chen2, Jing-Quan Yu1
1Department of Horticulture, Huajiachi Campus, Zhejiang University, Hangzhou, China.
Brassinosteroids (BRs) enhance plant stress tolerance by increasing reactive oxygen species (ROS). This suggests endogenous BRs play a crucial role in plant defense mechanisms against environmental challenges.
Area of Science:
- Plant Biology
- Molecular Biology
- Biochemistry
Background:
- Brassinosteroids (BRs) are key plant hormones regulating growth and development.
- Exogenous BR application improves plant tolerance to various stresses.
- Endogenous BRs' role in stress response requires further investigation.
Purpose of the Study:
- To investigate the role of endogenous brassinosteroids (BRs) in plant stress tolerance.
- To elucidate the mechanism of BR-induced stress tolerance, focusing on reactive oxygen species (ROS).
Main Methods:
- Field and greenhouse trials with exogenous BR application and BR biosynthesis inhibition.
- Assessing plant tolerance to photo-oxidative stress, cold stress, and cucumber mosaic virus.
- Measuring reactive oxygen species (ROS) accumulation and its sensitivity to NADPH oxidase inhibitors.
Main Results:
- Exogenous BR application enhanced stress tolerance, while BR biosynthesis inhibition compromised it.
- BR-induced stress tolerance is linked to increased ROS accumulation.
- ROS accumulation was sensitive to NADPH oxidase inhibitors, indicating their involvement.
Conclusions:
- Endogenous brassinosteroids (BRs) play a significant role in plant stress responses.
- Reactive oxygen species (ROS) mediate BR-induced stress tolerance, likely via gene regulation.
- ROS may also be involved in other BR-regulated processes like growth, development, and photosynthesis.
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