Increased glucose transfer in the rat jejunum after dietary potassium loading: effect of amiloride

The glucose transfer across the jejunum was measured in Wistar rats under a high potassium diet (HKD). In 12 of 27 HKD animals the transfer coefficient for D-glucose was not significantly higher than in control ones, (7.38 +/- 0.88).10(-5) s-1. In the other 15 a clear increase in glucose transfer was observed, (23.31 +/- 2.50).10(-5) s-1. The D-glucose transfer in the first group (n = 12) was, as in the case of the control rats, insensitive to amiloride section (10(-4) M), while D-glucose transfer became sensitive to amiloride in the second group (mean inhibition 94 +/- 8%, n = 14). A smaller but significant increase in L-glucose and sucrose transfers was also observed when the D-glucose movement was increased. No differences in short-circuit current, transepithelial potential, resistance and mucosa to serosa Na+ fluxes were observed between control and HKD rats and no effects of amiloride (10(-4) M) on these parameters were observed either in control or in HKD animals. [3H]Glucose uptake as also performed in brush-border vesicles prepared from rat jejunum, under control and HKD conditions. The specific and Na(+)-dependent 'overshoot' in D-glucose concentration, in vesicles prepared from HKD rats, became sensitive to amiloride action (10(-5) M). It is concluded that, besides the cellular adaptation induced in the distal portion of the nephron and large intestine, dietary potassium loading induces important modifications in glucose transfer in the rat jejunum.