Fluorescence modeling of droplets intersecting a focused laser beam
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Updated: Jun 13, 2026

Fluorescence detection methods for microfluidic droplet platforms
Published on: December 10, 2011
Bruno Frackowiak1, Cameron Tropea
1Institute for Fluid Mechanics and Aerodynamics, Technische Universität Darmstadt, Petersenstrasse 30, 64287 Darmstadt, Germany. Bruno.Frackowiak@sla.tu-darmstadt.de
Abstract:
Laser-induced fluorescence (LIF) has been previously used in various forms to characterize droplets in a spray, according to either size or temperature. A rigorous examination is presented of the LIF signal obtained when a water droplet seeded by Rhodamine 6G passes through one or two highly focused laser beams, i.e., with a beam waist of the order of the droplet diameter or smaller. The calculations are performed with a fluorescence model based on the generalized Lorenz-Mie theory and on ray tracing methods, assuming that the droplet is spherical and nonabsorbing. A parametric study reveals a size dependent signal as the droplet passes through the focused beam(s). The observed signal features suggest several avenues for the use of LIF scattering for size and velocity measurements of individual droplets.
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