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Updated: Jun 13, 2026
Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice
Published on: May 2, 2025
Perturbation in kidney lipid metabolic profiles in diabetic rats with reference to alcoholic oxidative stress
K R Shanmugam1, C H Ramakrishna, K Mallikarjuna
1Division of Molecular and Exercise Physiology, Department of Zoology, Sri Venkateswara University, Tirupati-517 502, A.P, India.
Abstract:
Diabetes is a major threat to global public health, and the number of diabetic patients is rapidly increasing worldwide. Evidence suggests that oxidative stress is involved in the pathophysiology of diabetic complications and alcoholic diseases. The aim of this study is to find out the impact of alcohol on lipid metabolic profiles in kidney tissue under streptozotocin induced diabetic condition. No study has been reported so far on the effect of alcohol on diabetic condition and also with reference to lipid metabolic profiles. Hence, the present study has been designed to elucidate the impact of alcoholism on diabetic condition. Male wistar strain albino rats were randomly divided into four groups: control (saline treated) NC, alcohol-treated (At), diabetic control (DC), and alcohol-treated diabetic rats (D+At). In alcohol-treated diabetic rats, we observed high levels of MDA, total cholesterol, triglycerides, phospholipids and also high levels of blood glucose than other groups. Moreover, degenerative changes of renal cells in alcohol-treated diabetic group were maximized by administration of alcohol as evinced by histopathological examination. This study suggests that alcohol consumption could be an aggravation factor which contributes for the formation of free radicals in diabetic condition. Therefore, consumption of alcohol during diabetic condition is harmful.
Insights
Alcohol consumption worsens kidney damage and oxidative stress in diabetic rats. This study highlights that drinking alcohol during diabetes is harmful, increasing risks associated with the disease.
Area of Science:
- Biochemistry
- Pathology
- Toxicology
Background:
- Diabetes mellitus poses a significant global health challenge, with increasing patient numbers worldwide.
- Oxidative stress is implicated in the development of diabetic complications and alcohol-related diseases.
- Limited research exists on the combined effects of alcohol and diabetes on lipid metabolism.
Purpose of the Study:
- To investigate the impact of alcohol consumption on lipid metabolic profiles within kidney tissue of rats with streptozotocin-induced diabetes.
- To elucidate the specific effects of alcoholism on the diabetic condition, particularly concerning renal lipid metabolism.
Main Methods:
- Male Wistar rats were divided into four groups: control, alcohol-treated, diabetic control, and alcohol-treated diabetic.
- Lipid profiles (total cholesterol, triglycerides, phospholipids) and malondialdehyde (MDA) levels were measured.
- Blood glucose levels were monitored, and histopathological examination of renal tissue was performed.
Main Results:
- Alcohol-treated diabetic rats exhibited significantly elevated levels of MDA, total cholesterol, triglycerides, and phospholipids compared to other groups.
- Increased blood glucose levels were observed in the alcohol-treated diabetic group.
- Histopathological analysis revealed maximized degenerative changes in renal cells of alcohol-treated diabetic rats.
Conclusions:
- Alcohol consumption exacerbates oxidative stress and negatively impacts lipid metabolism in the kidneys of diabetic rats.
- Alcohol acts as an aggravating factor in the free radical formation associated with diabetes.
- Combining alcohol consumption with diabetes is detrimental and increases health risks.
