High-performance liquid chromatographic method for the quantification of Mitragyna inermis alkaloids in order to
Veronique Sinou1, Julien Fiot, Nicolas Taudon
1UMR-MD3 Relations Hôte-Parasite, Pharmacologie et Thérapeutique, Unité de Recherche en Physiopathologie et Pharmacologie Parasitaire, Université de la Méditerranée, Institut de Recherche Biomédicale des Armées, Parc du Pharo, Marseille, France. veronique.sinou@club-internet.fr
Abstract:
In Africa, Mitragyna inermis (Willd.) O. Kuntze (Rubiaceae) is commonly used in traditional medicine to treat malaria. Antimalarial activity is mostly due to the hydromethanolic extract of M. inermis leaves and especially to the main alkaloids, uncarine D and isorhynchophilline. In the present study, we describe for the first time an HPLC method for the simultaneous quantification of uncarine D and isorhynchophylline in biological matrices. SPE was used to extract the components and the internal standard naphthalene from human and pig plasma samples. Chromatographic separation was performed on a C-18 reversed column at a flow rate of 1 mL/min, using methanol-phosphate buffer (10:90, pH 7), as a mobile phase. Good linearity was observed over the concentration ranges of 0.0662-3.31 microg/mL for uncarine D and 0.0476-2.38 microg/mL for isorynchophylline. The precision was less than 12% and the accuracy was from 86 to 107% without any discrepancy between the two species. Uncarine D and isorhynchophylline recoveries were over 80%. These results allowed the quantification of both uncarine D and isorhynchophylline in pig plasma after intravenous administration of M. inermis extract.
