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Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...

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Related Experiment Video

Updated: Jun 13, 2026

Live Imaging of Arabidopsis Pollen Tube Reception and Double Fertilization Using the Semi-In Vitro Cum Septum Method
06:45

Live Imaging of Arabidopsis Pollen Tube Reception and Double Fertilization Using the Semi-In Vitro Cum Septum Method

Published on: February 24, 2023

Molecular changes during pollen germination can be monitored by Raman microspectroscopy.

Franziska Schulte1, Ulrich Panne, Janina Kneipp

  • 1Humboldt Universität zu Berlin, Chemistry Department, Berlin, Germany.

Journal of Biophotonics
|May 4, 2010
PubMed
Summary

Pollen germination involves significant chemical changes within the pollen grain and developing pollen tube. Nutrient availability influences these metabolic shifts and molecular discharges, highlighting species-specific responses in Salix caprea and Fraxinus excelsior.

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Investigating Teliospore Germination Using Microrespiration Analysis and Microdissection
08:50

Investigating Teliospore Germination Using Microrespiration Analysis and Microdissection

Published on: May 13, 2018

Related Experiment Videos

Last Updated: Jun 13, 2026

Live Imaging of Arabidopsis Pollen Tube Reception and Double Fertilization Using the Semi-In Vitro Cum Septum Method
06:45

Live Imaging of Arabidopsis Pollen Tube Reception and Double Fertilization Using the Semi-In Vitro Cum Septum Method

Published on: February 24, 2023

Investigating Teliospore Germination Using Microrespiration Analysis and Microdissection
08:50

Investigating Teliospore Germination Using Microrespiration Analysis and Microdissection

Published on: May 13, 2018

Area of Science:

  • Plant reproductive biology
  • Molecular plant science
  • Biochemistry

Background:

  • Pollen germination is a critical step in plant reproduction.
  • Understanding the biochemical processes during germination is vital for plant science.
  • In vitro studies allow controlled investigation of germination factors.

Purpose of the Study:

  • To investigate the in vitro pollen germination of Salix caprea and Fraxinus excelsior.
  • To analyze the chemical composition changes during pollen germination.
  • To determine the influence of nutrient conditions on pollen germination and metabolism.

Main Methods:

  • In vitro pollen germination assays for Salix caprea and Fraxinus excelsior.
  • Spectroscopic analysis of pollen grain components (body vs. pollen tube).
  • Comparative analysis of germinated and ungermated pollen metabolism.

Main Results:

  • Significant chemical composition changes occur in pollen grains during germination.
  • Major chemical differences exist between the pollen grain body and the growing pollen tube.
  • Metabolic alterations are evident when comparing germinated and ungermated pollen.
  • Nutrient conditions impact the composition of germinating pollen and its units.
  • Species-specific utilization of stored metabolites and potential changes to the pollen outer coat were observed.
  • Molecular discharge into the medium is dependent on nutrient availability.

Conclusions:

  • Pollen germination involves dynamic biochemical transformations.
  • Nutrient availability plays a crucial role in regulating pollen germination processes and metabolic activity.
  • These findings have implications for future research on pollen dynamics and plant reproduction.