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Extraction: Advanced Methods00:56

Extraction: Advanced Methods

Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is formed in...

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Related Experiment Video

Updated: Jun 13, 2026

Extraction and Purification of Polyphenols from Freeze-dried Berry Powder for the Treatment of Vascular Smooth Muscle Cells In Vitro
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Removal of polyphenols from wine sludge using cloud point extraction.

Arhontoula Chatzilazarou1, Evangelos Katsoyannos, Olga Gortzi

  • 1Department of Oenology and Beverages Technology, Technological Educational Institution of Athens, Egaleo, Athens, Greece.

Journal of the Air & Waste Management Association (1995)
|May 5, 2010
PubMed
Summary

Cloud point extraction (CPE) efficiently recovers polyphenols from wine waste using Genapol X-080 or PEG 8000 surfactants. Recovered phenols retain significant antiradical activity, showcasing their potential value.

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Extraction and Purification of Polyphenols from Freeze-dried Berry Powder for the Treatment of Vascular Smooth Muscle Cells In Vitro
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Microwave-Assisted Extraction of Phenolic Compounds and Antioxidants for Cosmetic Applications Using Polyol-Based Technology
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Microwave-Assisted Extraction of Phenolic Compounds and Antioxidants for Cosmetic Applications Using Polyol-Based Technology

Published on: August 23, 2024

Area of Science:

  • Analytical Chemistry
  • Environmental Chemistry
  • Green Chemistry

Background:

  • Wine sludge is a significant waste product of the wine industry.
  • Polyphenols are valuable compounds with antioxidant properties.
  • Efficient recovery methods for polyphenols from waste streams are needed.

Purpose of the Study:

  • To investigate the use of cloud point extraction (CPE) for polyphenol recovery from wine sludge.
  • To optimize CPE parameters for maximum phenol recovery.
  • To assess the antiradical activity of recovered polyphenols.

Main Methods:

  • Cloud point extraction (CPE) using Genapol X-080 and PEG 8000 surfactants.
  • Optimization of parameters including surfactant concentration, temperature, and pH.
  • Antiradical activity assessment using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method.

Main Results:

  • Two-step CPE achieved 75.8% phenol recovery with 4% Genapol X-080 at pH 3.5 and 55°C.
  • Two-step CPE achieved 98.5% phenol recovery with 10% PEG 8000 at pH 2.5 and 55°C.
  • Recovered phenols demonstrated high antiradical activity.

Conclusions:

  • CPE is an effective and simple technique for recovering valuable polyphenols from wine sludge.
  • Genapol X-080 and PEG 8000 are suitable surfactants for polyphenol extraction.
  • The recovered polyphenols possess significant antioxidant potential, suggesting applications in various industries.