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Sheep genetic diversity in Bhutan using microsatellite markers
Tashi Dorji1, Han Jianlin, Po Wafula
1Renewable Natural Resources Research Centre, Jakar, Bhutan.
Animal Science Journal = Nihon Chikusan Gakkaiho
|May 5, 2010
Summary
Bhutanese sheep genetic diversity reveals two distinct clusters: Jakar-Sakten and Sipsu sheep. This genetic study recommends classifying these populations into at least two breeds based on molecular and phenotypic differences.
Area of Science:
- Animal Genetics
- Molecular Ecology
- Conservation Genetics
Background:
- Indigenous livestock breeds are crucial genetic resources.
- Understanding genetic diversity is vital for effective conservation and breed development.
- Bhutanese sheep populations represent a unique genetic heritage requiring characterization.
Purpose of the Study:
- To assess genetic diversity and population structure of five indigenous Bhutanese sheep.
- To investigate genetic relationships among Sakten, Jakar, Sarpang, Sipsu, and Tsirang sheep populations.
- To provide recommendations for the classification and conservation of Bhutanese native sheep breeds.
Main Methods:
- Genotyping using eight microsatellite markers.
- Analysis of genetic diversity parameters: observed and expected heterozygosities, mean number of alleles.
- Phylogenetic analysis including UPGMA tree and Principal Component Analysis (PCA).
Main Results:
- Jakar and Sakten populations exhibited the highest observed heterozygosities, while Tsirang showed the lowest.
- Genetic analyses indicated a close relationship among Tsirang, Sipsu, and Sarpang populations.
- Jakar and Sakten populations formed a distinct cluster, separate from the Tsirang-Sipsu-Sarpang cluster, with geographical and phenotypic distinctions.
Conclusions:
- Bhutanese native sheep populations can be broadly classified into at least two distinct groups: Jakar-Sakten sheep and Sipsu sheep.
- Distinct molecular and phenotypic characteristics support the proposed breed classification.
- Further research is needed to elucidate genetic differences between Jakar and Sakten sheep due to their differing phenotypes.
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