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Related Concept Videos

Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...

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Related Experiment Video

Updated: Jun 13, 2026

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method
09:32

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method

Published on: September 10, 2017

Advances in capillary electrophoretic enzyme assays.

Yi Fan1, Gerhard K E Scriba

  • 1Department of Pharmaceutical Chemistry, School of Pharmacy, University of Jena, Philosophenweg 14, D-07743 Jena, Germany.

Journal of Pharmaceutical and Biomedical Analysis
|May 5, 2010
PubMed
Summary

Capillary electrophoresis (CE) offers efficient enzyme assays for characterization and analysis. This review covers CE-based enzyme assays and microfluidic applications from 2006-2009.

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Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System
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Published on: November 21, 2023

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Last Updated: Jun 13, 2026

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method
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Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method

Published on: September 10, 2017

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System
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Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Published on: November 21, 2023

Area of Science:

  • Analytical Chemistry
  • Biochemistry

Background:

  • Capillary electrophoresis (CE) is increasingly utilized for enzyme assays.
  • CE offers high separation efficiency, rapid analysis, and minimal sample/reagent consumption.

Purpose of the Study:

  • To review literature on CE-based enzyme assays from 2006 to November 2009.
  • To highlight applications of microfluidic devices in CE enzyme assays.

Main Methods:

  • Categorization of CE enzyme assays into pre-capillary (offline) and online methods.
  • Online assays involve enzyme reaction and separation within the same capillary, using immobilized or solution-phase enzymes (EMMA).

Main Results:

  • CE applications encompass enzyme activity determination, substrate/modulator characterization, and metabolic pathway investigation.
  • Microfluidic device applications are also discussed.

Conclusions:

  • CE is a versatile tool for comprehensive enzyme analysis.
  • The review provides a focused overview of recent advancements in CE enzyme assay techniques.