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Updated: Jun 13, 2026

Assaying the Kinase Activity of LRRK2 in vitro
Published on: January 18, 2012
Arsenic inhibits neurite outgrowth by inhibiting the LKB1-AMPK signaling pathway
Xin Wang1, Dan Meng, Qingshan Chang
1Graduate Center for Toxicology, University of Kentucky, Lexington, Kentucky 40536 , USA.
Background:
Arsenic (As) is an environmental pollutant that induces numerous pathological effects, including neurodevelopmental disorders.
Objectives And Methods:
We evaluated the role of the LKB1-AMPK pathway in As-induced developmental neurotoxicity using Neuro-2a (N2a) neuroblastoma cells as a model of developing neurons.
Results:
The addition of low concentrations of As (
Conclusions:
Reduced neurite outgrowth induced by As results from deficient activation of AMPK as a consequence of a lack of activation of LKB1. Oxidative stress induced by As, especially excessive superoxide, plays a critical role in blocking the LKB1-AMPK pathway. Our studies provide insight into the mechanisms underlying As-induced developmental neurotoxicity, which is important for designing a new strategy for protecting children against this neurotoxic substance.
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