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Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Related Experiment Video

Updated: Jun 13, 2026

Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos
10:15

Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos

Published on: April 28, 2017

Digital scanned laser light sheet fluorescence microscopy.

Philipp J Keller, Ernst H K Stelzer

    Cold Spring Harbor Protocols
    |May 5, 2010
    PubMed
    Summary
    This summary is machine-generated.

    Digital scanned laser light sheet fluorescence microscopy (DSLM) offers a novel solution for quantitative in vivo imaging. This technique addresses key challenges in imaging large specimens with high resolution and speed, crucial for life science applications.

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    Simultaneous Live Imaging of Multiple Insect Embryos in Sample Chamber-Based Light Sheet Fluorescence Microscopes

    Published on: September 9, 2020

    Area of Science:

    • Life Sciences
    • Biomedical Imaging
    • Microscopy

    Background:

    • In vivo imaging is critical for modern life sciences, often requiring cellular or subcellular resolution in large organisms.
    • Existing light microscopy techniques face challenges in balancing imaging speed, phototoxicity, resolution, and signal-to-noise ratio for complex biological samples.
    • Multiple-view imaging is essential for overcoming light scattering and absorption in thick biological specimens.

    Purpose of the Study:

    • To introduce and discuss digital scanned laser light sheet fluorescence microscopy (DSLM) as a novel tool for quantitative in vivo imaging.
    • To evaluate DSLM's capabilities in addressing the conflicting requirements of high-speed, high-resolution, low-phototoxicity imaging.
    • To compare DSLM with established 3D microscopy techniques like confocal and two-photon microscopy.

    Main Methods:

    • Digital scanned laser light sheet fluorescence microscopy (DSLM) is presented as the core imaging technique.
    • The discussion involves comparing DSLM's performance against confocal fluorescence microscopy and two-photon microscopy.
    • Focus on quantitative in vivo imaging in the post-genomic era.

    Main Results:

    • DSLM demonstrates potential to fulfill the demanding requirements for in vivo imaging of large, complex biological specimens.
    • The technique offers a balance of high imaging speed, low photobleaching, low phototoxicity, and good 3D resolution.
    • DSLM's multiple-view imaging capability is highlighted as crucial for scattering or absorbing tissues.

    Conclusions:

    • DSLM emerges as a powerful and versatile tool for quantitative in vivo imaging in the post-genomic era.
    • This technique is essential for applications such as studying fast cellular dynamics, whole embryo development, and morphological defect analysis.
    • DSLM represents a significant advancement over current 3D microscopy methods for specific biological imaging challenges.