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Updated: Jun 13, 2026

Mixed Primary Cultures of Murine Small Intestine Intended for the Study of Gut Hormone Secretion and Live Cell Imaging of Enteroendocrine Cells
Published on: April 20, 2017
Nutrient regulation of enteroendocrine cellular activity linked to cholecystokinin gene expression and secretion
K N Nilaweera1, L Giblin, R P Ross
1Food for Health Ireland, Teagasc, Moorepark Food Research Centre, Fermoy, County Cork, Ireland. Kanishka.nilaweera@teagasc.ie
Abstract:
The hormone cholecystokinin is produced by the enteroendocrine I cells in the intestine, and it plays an important role in a number of physiological processes including digestion and food intake. Recent data suggest that cholecystokinin gene expression and protein secretion are regulated by macronutrients. The mechanism involves a change in intracellular levels of cAMP and Ca(+2), brought about by the activity of a number of nutrient-responsive G protein-coupled receptors, nutrient transporters, ion channels and intracellular enzymes. How these intracellular responses could lead to gene expression and protein secretion are discussed along with new directions for future investigation.
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