Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell Migration01:19

Cell Migration

Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
Cell Migration01:09

Cell Migration

Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
Role of Myosin in Cell Migration01:18

Role of Myosin in Cell Migration

Myosins are multimeric motor proteins involved in various cellular processes such as migration, adhesion, and proliferation. Myosin II is the most common type in animal cells, which binds and cross-links actin filaments.
Myosin II  is a hexamer comprising two heavy chains with globular heads and coiled-coil tails, two regulatory light chains, and two essential light chains. The ATPase sites on the myosin heads hydrolyze ATP, and the released phosphate generates the force for contraction. It is...
Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker proteins that...
Mechanism of Lamellipodia Formation01:31

Mechanism of Lamellipodia Formation

Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
Gastrulation01:56

Gastrulation

Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata will form...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Migrating Epithelial Monolayer Flows Like a Maxwell Viscoelastic Liquid.

Physical review letters·2020
Same author

Author Correction: Spontaneous shear flow in confined cellular nematics.

Nature physics·2019
Same author

Spontaneous shear flow in confined cellular nematics.

Nature physics·2018
Same author

Turbulent Dynamics of Epithelial Cell Cultures.

Physical review letters·2018
Same author

Characterization of a Biomimetic Mesophase Composed of Nonionic Surfactants and an Aqueous Solvent.

Langmuir : the ACS journal of surfaces and colloids·2016
Same author

The mechanotransduction machinery at work at adherens junctions.

Integrative biology : quantitative biosciences from nano to macro·2015

Related Experiment Video

Updated: Jun 13, 2026

Quantitative Assessment of Human Neutrophil Migration Across a Cultured Bladder Epithelium
11:20

Quantitative Assessment of Human Neutrophil Migration Across a Cultured Bladder Epithelium

Published on: November 7, 2013

Velocity fields in a collectively migrating epithelium.

L Petitjean1, M Reffay, E Grasland-Mongrain

  • 1Laboratoire Physico-chimie Curie, Institut Curie, Centre de Recherche, CNRS, Université Pierre et Marie Curie, Paris, France.

Biophysical Journal
|May 6, 2010
PubMed
Summary

This study quantifies cell migration velocity fields in epithelia using particle image velocimetry. Madin-Darby canine kidney (MDCK) cells show more coherent migration than normal rat kidney (NRK) cells.

More Related Videos

Study of Cell Migration in Microfabricated Channels
09:36

Study of Cell Migration in Microfabricated Channels

Published on: February 21, 2014

Traction Microscopy Integrated with Microfluidics for Chemotactic Collective Migration
10:53

Traction Microscopy Integrated with Microfluidics for Chemotactic Collective Migration

Published on: October 13, 2019

Related Experiment Videos

Last Updated: Jun 13, 2026

Quantitative Assessment of Human Neutrophil Migration Across a Cultured Bladder Epithelium
11:20

Quantitative Assessment of Human Neutrophil Migration Across a Cultured Bladder Epithelium

Published on: November 7, 2013

Study of Cell Migration in Microfabricated Channels
09:36

Study of Cell Migration in Microfabricated Channels

Published on: February 21, 2014

Traction Microscopy Integrated with Microfluidics for Chemotactic Collective Migration
10:53

Traction Microscopy Integrated with Microfluidics for Chemotactic Collective Migration

Published on: October 13, 2019

Area of Science:

  • Cell biology
  • Biophysics
  • Tissue engineering

Background:

  • Cell migration is crucial for development and wound healing.
  • Understanding collective cell migration dynamics is key to tissue regeneration.
  • Previous studies lacked quantitative analysis of velocity fields in motile epithelia.

Purpose of the Study:

  • To quantitatively measure the velocity field of collectively migrating cells in a motile epithelium.
  • To analyze key parameters like velocity module, order parameter, and velocity correlation function.
  • To compare the migration behavior of Madin-Darby canine kidney (MDCK) cells and normal rat kidney (NRK) cells.

Main Methods:

  • Microstencil technique to initiate controlled migration without cell damage.
  • Particle image velocimetry (PIV) to map the velocity field, avoiding single-cell tracking challenges.
  • Biorthogonal decomposition of the velocity field for coherence analysis.

Main Results:

  • MDCK cells exhibited a sharp velocity decrease post-confluence, unlike NRK cells.
  • Measured velocity correlation length: ~200 microm for MDCK cells vs. ~40 microm for NRK cells.
  • MDCK cell velocity fields were significantly more coherent than NRK cells.

Conclusions:

  • MDCK cells display highly coherent collective migration patterns.
  • The migration behavior differs significantly between MDCK and NRK cell types.
  • Cellular coherence influences tissue-level migration dynamics over considerable distances.