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A new mediator method for BOD measurement under non-deaerated condition
Ling Liu1, Li Shang, Chang Liu
1State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China.
Talanta
|May 6, 2010
Summary
A new non-deaerated biochemical oxygen demand (BOD) method using ferricyanide offers a simpler, faster alternative for water quality monitoring. This approach, termed NDA-BOD(Med), avoids oxygen removal, making it practical for real-world applications.
Area of Science:
- Environmental Chemistry
- Analytical Chemistry
- Microbiology
Background:
- Traditional mediator methods for biochemical oxygen demand (BOD) monitoring require deaeration, posing practical challenges.
- Existing BOD(Med) methods are limited by the need for oxygen-free conditions, hindering field applications.
Purpose of the Study:
- To develop and validate a non-deaerated BOD mediator (NDA-BOD(Med)) method for practical water quality analysis.
- To assess the feasibility of using ferricyanide as an electron acceptor in a non-deaerated system with Escherichia coli.
Main Methods:
- Utilized ferricyanide as an electron acceptor, reduced to ferrocyanide by Escherichia coli catalyzing organic substrates.
- Compared biodegradation efficiencies under deaerated and non-deaerated conditions.
- Investigated the role of oxygen as a natural electron acceptor and endogenous oxygen consumption.
Main Results:
- Similar biodegradation efficiencies were observed in both deaerated and non-deaerated conditions.
- Oxygen concentration was significantly lower than the mediator, minimizing its interference.
- Oxygen consumption in NDA-BOD(Med) was primarily due to endogenous bacterial activity.
Conclusions:
- The NDA-BOD(Med) method is feasible and effective, offering a simplified approach to BOD determination.
- This method is suitable for rapid BOD measurements, especially for low-concentration samples and in situ water monitoring.
- NDA-BOD(Med) presents an advantageous alternative for practical environmental water analysis.
