Amperometric glucose biosensor based on biocompatible poly(dimethylaminoethyl) methacrylate microparticles

Area of Science:

• Biochemistry
• Materials Science
• Analytical Chemistry

Background:

• Glucose oxidase (GOx) is crucial for glucose detection.
• Developing stable and sensitive biosensors remains a key challenge.
• Poly(dimethylamino) ethyl methacrylate (p-DMAEM) offers a promising matrix for enzyme immobilization.

Purpose of the Study:

• To immobilize glucose oxidase (GOx) within p-DMAEM microparticles for glucose biosensor fabrication.
• To optimize the immobilization process by varying monomer concentration and cross-linker content.
• To characterize the analytical performance and stability of the developed glucose biosensor.

Main Methods:

• Enzyme immobilization of GOx within p-DMAEM microparticles.
• Optimization of microparticle synthesis parameters (monomer concentration, cross-linker content).
• Characterization of biosensor performance (working potential, pH, temperature, enzyme loading, sensitivity, linear range, response time, detection limit).

Main Results:

• Optimized microparticles synthesized with 1.19M monomer and 0.37% cross-linker content showed the best biosensor response.
• Key analytical properties like sensitivity, linear range, and detection limit were determined.
• The biosensor demonstrated satisfactory performance in analyzing glucose in human serum samples.
• The biosensor exhibited a long useful lifetime of at least 520 days.

Conclusions:

• p-DMAEM microparticles provide an effective matrix for GOx immobilization, leading to a robust glucose biosensor.
• The developed biosensor offers high sensitivity, stability, and a long operational lifetime.
• This approach holds significant potential for accurate glucose monitoring in clinical diagnostics.